| 1840—175 years | Cherry Lake | NW |
| Lake City, First | NW | |
| 1865—150 years | Hastings, St. Stephens | NE |
| Lake Butler, First | NE | |
| Live Oak, First | NW | |
| Kissimmee, Shingle Creek | EC | |
| 1890—125 years | Arredondo, Wesley Chapel | NC |
| Haines City, New Horizon | SC | |
| Jacksonville, Riverside Park | NE | |
| Jennings | NW | |
| Old Town | NW | |
| Sanford, Christ | EC | |
| Seminole, First | GC | |
| 1915—100 years | Davie | SE |
| DeLeon Springs | EC | |
| Dunedin, First | GC | |
| Englewood | SW | |
| Lake Panasoffkee | NC | |
| New Port Richey, First | GC | |
| Okeechobee, First | AC | |
| 1940—75 years | Gainesville, Wesley | NC |
| Plant City, Springhead | SC | |
| 1965—50 years | East Naples | SW |
| Ft. Lauderdale, Harris Chapel | SE | |
| Orange Park, Calvary | NE | |
| Rockledge | AC | |
| Sebring, St. John | SW | |
| Steinhatchee | NW | |
| Tallahassee, Gary Memorial | NW | |
| Vero Beach, Christ by the Sea | AC | |
| West Palm Beach, Good Shepherd | AC | |
| 1990—25 years | Boynton Beach, Faith | AC |
| Cape Coral, Hope | SW | |
| Merritt Island, North Merritt Island | AC | |
| Miami, Iglesia Christiana Juan Wesley | SE | |